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A release call is produced by a male toad or an unreceptive female toad when a frog or other animal (including a human hand) grabs it across the back in the position used for mating or amplexus. It's a frog's way of saying "Get off my back! Let go!" In some species of toads, this call is also made during the breeding season when a male toad enters another male toad's territory.
The Amargosa Toad is only known to produce a release call.
This is a 14 second recording of the release calls of an Amargosa Toad (sex not determined) made at night in Nye County, Nevada. Insects are heard in the background.